Abstract

Dadih is a traditional fermented buffalo milk consumed by the Minangkabau people in West Sumatera Province, Indonesia, especially in Bukittinggi, Padang Panjang, Solok, Lima Puluh Kota, and Tanah Datar districts. The objective of this study was to identify amino acid and strain of acid lactic bacteria isolated from dadih and to evaluate the benefit of dadih intervention during pregnancy on the nutritional status of an infant's birth. This study was initiated with DNA extraction from bacterial cultures, Ribosomal DNA amplification, and sequencing, analysis of amino acid using UPLC. The intervention was conducted in two districts on 138 pregnant mothers for 6 months. A prospective cohort study was conducted on a follow up of a previous randomized controlled trial. This study was conducted in two districts in West Sumatera Province from April 2018 to July 2019. Spontaneous probiotic species of dadih strain were identified in this study: Lactobacillus Plantarum, Lactobacillus casei, Lactobacillus durans, Leuconostoc pseudo mesentoroides, and Lactobacillus cactis. Glutamic acid was the highest quantity of non-essential amino acid in dadih (16.28 mg/g), while Lysine and Leucine were the highest essential amino acids (7.22 and 6.42 mg/g). The study revealed that after a 6-month intervention, about 66.7% of infants birth weight > 3000 grams and only 2.1% of infants having birth weight < 2500 grams for Dadih Group and about 60.0% had infant birth weight > 3000 grams and 4.4% infants with low birth in Control Group. The result of this study shows that there are more benefits in pregnant mothers' weight gain and infants' birth weight until a six-month follow up from pregnant mothers in Dadih Group than Control Group. The results of the follow-up study show that infants from mothers supplemented with Dadih Zinc Group have significantly less undernutrition status as compared to the Control Group (p<0,05). The results also show that dadih has a sufficient amount of protein to fulfill the nutritional need for pregnant mothers and the outcomes.

Highlights

  • Dadih is a traditional food that is popular in several West Sumatra districts, such as Bukittinggi, Padang Panjang, Solok, Lima Puluh Kota, and Tanah Datar district

  • The result of this study shows that there are more benefits in pregnant mothers' weight gain and infants' birth weight until a six-month follow up from pregnant mothers in Dadih Group than Control Group

  • The results show that dadih has a sufficient amount of protein to fulfill the nutritional need for pregnant mothers and the outcomes

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Summary

Introduction

Dadih is a traditional food that is popular in several West Sumatra districts, such as Bukittinggi, Padang Panjang, Solok, Lima Puluh Kota, and Tanah Datar district. It is important to reintroduce dadih to the community by using different methods, such as a bamboo cup as its packaging. For this purpose, many kinds of bamboo can be used, such as Talang bamboo and Buluah Lapoh. Dadih makers still use traditional ways to produce dadih. Sometimes they do not pay much attention to sanitation and hygiene while using a non-pasteurized process and unclean bamboo cups. Because this rather unsafe process has been passed down from generation to generation, gjhs.ccsenet.org

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