Characteristics of a recombinant Lentinula edodes endoglucanase and its potential for application in silage of rape straw

Abstract

An experiment was conducted to determine the characteristics of recombinant endoglucanase and its effects on rape straw silage. The endoglucanase from Lentinula edodes (LeCel12A) was produced in Pichia pastoris and shown maximum activity at 40 °C and pH 3.0. The LeCel12A exhibited preferential hydrolysis of carboxymethylcellulose. The activity of LeCel12A could be enhanced by MnCl2 in dose-dependent manners. Trp22 was a key amino acid affecting LeCel12A activity. The LeCel12A enhanced the hydrolysis of rape straw, rice straw, wheat straw, and corn straw. Supplemental LeCel12A increased lactic acid concentration and reduced lignocellulosic content of the rape straw silage. Though an increase in the saccharification efficiency of LeCel12A-treated rape straw silage was observed when the fibrolytic enzyme loading of hydrolysis system was enough, supplemental LeCel12A did not dramatically enhance the saccharification of rape straw silage in the current study. This study demonstrates that LeCel12A may be useful for improving the utilization of rape straw silage as an additive, but its supplemental dose, cost benefit, and consequent application possibility in biofuel production require careful consideration and further investigation.