Abstract
A new reo-like virus was isolated from landlocked salmon around the Wu-Lin farm in the upper basin of Ta-Chia river, and designated as LSV (landlocked salmon virus). It has shown typical plaque-like syncytial CPE in tissue culture, and replicated very well in AS, BF-2, BB, CCO and CHSE-214 cell lines. But RTG-2 and TO-2 cells produced little virus. The optimal growth temperature of LSV in CHSE-214 is 20°C. The typical double-layered capsid of icosahedral virion with a 78 nm diameter was shown in electron micrography. The buoyant density of LSV in CsCl is 1.365g/ml. Those are typical characteristics of Reoviridae. However, LSV has 11 segmented ds RNA genomes and 5 major virion proteins, has aquatic animal hosts which are characteristics of aquareovirus, but is different from viruses of the defined six genera of Reoviridae. Compared to the electrophoretype of RNA genomes and virion proteins of LSV, CSV, CRV, GSV, and 13P2, the RNA pattern of LSV is closer to that of CSV, but the virion protein pattern is closer to that of 13P2. However, each aquareovirus has its own unique RNA and virion protein pattern. These results indicate that LSV is a newly discovered virus and the first aquareovirus isolated in Taiwan.
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