Abstract
It is important to understand the differences in characteristics between plasmid-harboring recombinant yeast and chromosome-integrated recombinant yeast for the design and optimization of bioprocess employing recombinant yeast. In the present study, heterologous glucoamylase gene was inserted into yeast. The glucoamylase activity per gene copy number of chromosome-integrated recombinant yeast MMY2SUCSTA-I-5 was 3 to 6 folds higher than that of plasmid-harboring recombinant yeast MMY2SUCSTA. And the genetic stability of chromosome-integrated recombinant yeast (99%) was far better compared to plasmid-harboring recombinant yeast (65%). Better genetic stability and glucoamylase activity per gene copy number of chromosome-integrated recombinant yeast can provide advantages in higher final expression level, especially in continuous culture, compared to the plasmid-harboring recombinant yeast. The optimal glucose concentration for maximum expression of glucoamylase in chromosome-integrated recombinant yeast was lower than that in plasmid-harboring recombinant yeast.
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