Abstract
ObjectivesWe aimed to construct an induction system for polyploid giant cancer cells (PGCCs), as well as to investigate PGCC features and clinical significance.MethodsA laryngeal neoplasm‐PGCC induction system was constructed using paclitaxel liposomes (PTX). We used western blots to compare expression of epithelial‐mesenchymal transition‐related proteins, stem cell interrelated proteins, and cyclin‐associated proteins. We then measured PGCC count in tissue samples of patients with laryngeal neoplasms and analyzed its relationship with prognosis. Statistical significance was determined using t‐tests.ResultsPTX successfully induced PGCCs. Western blotting showed that CyclinB1, CDC25C, CDK1, E‐cadherin, and EIF‐4A expression decreased in PGCCs compared with normal cancer cells, whereas vimentin and CD133 expression increased. Number of PGCCs in laryngeal cancer tissues and overall survival time were inversely correlated (P < .05).ConclusionsPTX successfully induces PGCC formation in laryngeal carcinoma, which may be the cause of poor prognosis in patients with laryngeal cancer.Level of Evidence: 4.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.