Characteristic Study of the Marine Algae &lt;i&gt;Sargassum sp&lt;/i&gt;. on Metal Adsorption

Saravanan Saravanan

doi:10.3844/ajassp.2011.691.694

Abstract

Problem statement: Biomass of brown marine macro algae is a biological resource that is available in large quantities and can form a good base for the development of biosorbent material. Approach: Algae have been found to be potentially suitable biosorbents because of its cheap availability, both in fresh or salt water, relatively high surface area and high binding affinity. Results: The study considered the molecular status of the biomass such as DNA, Protein and Pigment analysis on the adsorption of metal from effluent. The Molecular studies showed that DNA, protein and chlorophyll content are greatly affected because of metal adsorption. Conclusion/Recommendations: The Activesite prediction showed the binding site of the metal on the biomass. Based on the results obtained it can be concluded that the biomass Sargassum sp. has higher affinity towards the adsorption of metals.

Highlights

The collected samples were rinsed with distilled waterBiomass of brown marine macro algae is a biological resource that is available in large quantities for the removal of external salts and sand and with acetone solution.and can form a good base for the development of biosorbent material (Chu et al, 1997)
The isolated DNA of both the fresh and metal adsorbed biomass samples were run in agarose gel electrophoresis and bands were observed under uvtransilluminator
The chlorophyll and carotene test were undergone to three-dimensional (3D) structure determines protein function

Summary

INTRODUCTION

Biomass of brown marine macro algae is a biological resource that is available in large quantities for the removal of external salts and sand and with acetone solution. The DNA was isolated from fresh and metal adsorbed biomass. To isolate the DNA the biomass was ground with lysis to occur through interactions with the cell walls This is buffer (2% Sarcosyl, 10mM EDTA (pH 8.0), 0.1M due to the presence of various functional groups such as carboxyl, amino, sulphate and hydroxyl groups, which can act as binding agents, include ionic interactions and complex formation between metal cations and ligands on the surface of the seaweeds. Sample collection: Fresh samples of brown marine extracted twice with an equal volume of 24:1 macro alga, Sargassum sp. 1gram of fresh/metal adsorbed algae was weighed and homogenized in mortar and pestle with 10ml of water It was mixed with 4.5 mL of 80% acetone and was centrifuged at 8,000rpm for 5mins. The supernatant was collected and used for the measurement of optical density at different wavelength such as 645, 663, 638 and 490 nm respectively (Arnon, 1949)

AND DISCUSSION

CONCLUSION