Abstract
In current study plasmids (size from 6.2 to 8.5 kb) copied with the “rolling circle” mechanism (RCR type) of the pC194 family have been identified in environmental bacteria Bacillus pumilus. It is shown that these extrachromosomal elements are widely distributed in B. pumilus bacteria circulating on the territory of Belarus (19 strains from 41 contain RCRplasmids) and they are characterized by genetic polymorphism. The most common extrachromosomal genetic elements (7.7 kb size) are identical to the plasmid pBp15.1S from the entomopathogenic strain B. pumilus 15.1. The remaining 6 type of plasmids differ from each other and from the known extrachromosomal genetic elements of genus Bacillus. In the investigated bacteria no replicons similar to pLS20 were detected.
Highlights
Host function specified by Bacillus pumilus plasmid pPL7065 / P
Draft genome sequence of Bacillus pumilus strain GM3FR, an endophyte isolated from aerial plant tissues of Festuca rubra L. / J
Use of a plasmid DNA probe to monitor populations of Bacillus pumilus inoculant strains in hay / C
Summary
Для установления классификационной принадлежности выявленных плазмид определяли сходство их нуклеотидных последовательностей с известными внехромосомными элементами бактерий рода Bacillus. Сиквенс-анализ встроенных участков ДНК позволил установить их сходство с плазмидами RCR-типа бактерий B. pumilus, B. subtilis и B. safensis. Н. плазмиды рВР-В171 с целым рядом плазмид B. pumilus (pBp15.1S, pGR8, pPDSLzg-1, pC2-2, pPL7065) и плазмидой pPOD2000 бактерий B. subtilis. Н. плазмиды рВР-6322 проявлял сходство с другими плазмидами бактерий B. safensis (pBA64) и B. pumilus (pBP6000 и рGLB197) Н. pGR8, B. pumilus pDW5-4, B. zhangzhouensis pBp15.1S, B. pumilus pPDSLzg-1, B. pumilus pPL10, B. pumilus pPOD2000, B. subtilis рВР-В171, 838 п. Н. pBp15.1S, B. pumilus pGR8, B. pumilus pPOD2000, B. subtilis pPDSLzg-1, B. pumilus pC2-2, B. pumilus pPL7065, B. pumilus рВР-33-3, 840 п. Н. pGR8, B. pumilus pBp15.1S, B. pumilus pDW5-4, B. zhangzhouensis pPDSLzg-1, B. pumilus pPL10, B. pumilus pPZZ84, B. pumilus рВР-6322, 813 п. Сходные последовательности в ГенБанке рВР-Т2, 755 п. н. pGR8, B. pumilus pDW5-4, B. zhangzhouensis pBp15.1S, B. pumilus pPDSLzg-1, B. pumilus pPL10, B. pumilus pPOD2000, B. subtilis рВР-В171, 838 п. н. pBp15.1S, B. pumilus pGR8, B. pumilus pPOD2000, B. subtilis pPDSLzg-1, B. pumilus pC2-2, B. pumilus pPL7065, B. pumilus рВР-33-3, 840 п. н. pGR8, B. pumilus pBp15.1S, B. pumilus pDW5-4, B. zhangzhouensis pPDSLzg-1, B. pumilus pPL10, B. pumilus pPZZ84, B. pumilus рВР-6322, 813 п. н. pBA64, B. safensis pBP6000, B. pumilus рGLB197, B. pumilus
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More From: Proceedings of the National Academy of Sciences of Belarus, Biological Series
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