CHARACTERISTIC OF INNATE COMPONENT OF LOCAL IMMUNITY OF IN PERIODONTIUM TISSUES OF RATS AFTER TOOTH EXTRACTION IN DIABETES MELLITUS BY CD 68 EXPRESSION INDICES

Abstract

CD 68 is conventionally used as a highly specific cytochemical marker for monocytes / macrophages immune staining during histochemical analysis of tissues with suspected inflammatory or timorous process. Therefore, the aim of this study was to evaluate the peculiarities of the status of an innate component of local immunity in the periodontium tissues by using CD68 expression indices in rats with modelled streptozotocin-induced diabetes on the 1st, 7th and 14th days after extraction of the first lower molar. Materials and methods. Research was conducted on 120 Wistar male rats aged 8-10 months, divided into 8 groups of 15 animals in each. There were four control groups "Control-0", for which no tooth extraction was performed on; “Control-1; -7 and -14" groups involved the rats, whose parameters were registered on the 1st, 7th and 14th day after the extraction; and four relevant groups with modelled diabetes mellitus (simulated by intraperitoneal introduction of streptozotocin (SIGMA Chemical, the USA) in a dose of 50 mg/kg), on the 21st day of diabetes, the average glycemic level in the groups was 22.65 ± 0.88 mM/L. The tooth extraction was performed under thiopental anaesthesia (in a dose 40 mg / kg) with additional local infiltration anaesthesia using "Ubisthesin" (3M Deutschland GmbH, Germany). In order to study CD68 expression indices, dewaxed and rehydrated periodontium cross sections were incubated with the monoclonal antibodies: primary rabbit IgG to CD68 (Santa Cruz Biotechnology, the USA) using 1:200 dilution and secondary mouse IgG to the complete molecule of rabbit IgG conjugated with FITC (Santa Cruz Bioteology) using 1:200 dilution. Results. The tooth extraction in rats with normal values of carbohydrate metabolism leads to the development of inflammation, macrophage infiltration in periodontal soft tissues on the 7th day, and inflammation "fading" following the second week. Modelled diabetes mellitus contributes to the formation of a “hidden” inflammation with the extended macrophage infiltration in the periodontal soft tissues. Thus, in diabetes mellitus, the tooth extraction slightly increases the expression of CD 68, the process slowly progresses, but is characterized by prolongation in the long-term period.