Abstract

Bio-H2 production from organic wastewater together with lignocellulose wastes not only achieved the H2 energy recovery, but also be beneficial to carbon emission reduction and carbon neutralization. In order to obtain higher energy recoveries, promotion attempts were performed in bio-H2 fermentation with low temperature (−80–0 °C) pretreated peanut shell powder (PSP) as co-substrate. A maximum H2 production of 109.2 mL was obtained as almost double of the sum from the same amount of untreated PSP and glucose as sole substrate. The enhancement was co-contributed by 44% from PSP supplementary, 35% from low-temperature pretreatment, and 2.8% from buffer effect and acidification, respectively, and realized through C/N balancing, PSP conversion influencing, fermentative pH buffering and time prolonging. The experimental results uncovered the co-contribution realization ways of supplementing low-temperature pretreated lignocellulose wastes in the bio-H2 fermentation system, and provided mechanism support for application potential of low-temperature pretreatment on lignocellulose wastes in cold regions.

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