Abstract
The objectives of this research was to evaluate polymorphism of microsatellite DNA through the investigation of loci BM1824, SPS115, and ILSTS028, and their possible association with body weight of Aceh cattle. A total of 121 of DNA samples were collected from Aceh cattle population in Indrapuri. Genetic polymorphism of Aceh cattle was calculated in frequency of alleles and genotypes. Association between genotypes and body weight was calculated by general linear model (GLM). Result showed that three loci showed high polymorphism. BM1824 locus had 11 genotypes with 3 highest frequency for BC (30.87), CC (17.28) then BB (14,82), while frequency of the other 8 genotypes namely : AB, AC, AD, AF, BD, BE, BF, and CD were less than 10 %. SPS115 locus had 9 genotypes with two highest frequency for CE (30.00) and BE (20.00), while the other 7 genotype were less than 15%. ILSTS028 locus had 22 genotypes with two highest frequencies for CH (24.69) and CC (13.59) respectively. It is conduded that Aceh cattle possessing typical genotypes of BE in BM1824 locus, AE in SPS115, and BG in ILSTS028 locus was apparently related to a higher body weight compared to other genotypes. On the contrary, those possessing AB in BM1824 and CK genotypes in ILSTS028 were seemingly associated with a lower body weight. Key Words : Aceh Cattle, Microsatellite, Body Weight
Highlights
JLLFGR (Joint Laboratory on Livestock and Forage Genetic Resources) Beijing-China 3Balai Pembibitan Ternak Unggul (BPTU) Sapi Aceh, Indrapuri, Banda Aceh (Diterima 3 Juni 2012 ; disetujui 20 Agustus 2013)
Genetic polymorphism of Aceh cattle was calculated in frequency of alleles and genotypes
Association between genotypes and body weight was calculated by general linear model (GLM)
Summary
Ekstraksi DNA genom dilakukan dengan metoda Sambrook et al (1989) yang telah dimodifikasi dengan menggunakan buffer lisis sel DNA dimurnikan dengan metode fenol-kloroform, yaitu dengan menambahkan 40 μl 5 M NaCl dan 400 μl fenol dan kloroform iso amil alkohol (CIAA). DNA diendapkan dengan 40 μl 5 M NaCl dan 800 μl etanol absolute. Endapan dicuci dengan menambahkan 800 μl 70% etanol, disentrifugasi dengan kecepatan 12 000 rpm selama 5 menit, etanol dibuang dan diuapkan, selanjutnya DNA dilarutkan dengan 100 μl 80% TE (Elution buffer)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
