Abstract
A set of European obsolete wheat cultivars and landraces was evaluated using molecular markers. Three primer pairs were used to amplify LMW-GS coding sequences. To enrich the current gene-pool, modern Czech wheat cultivars were used for the assessment of the potential of old genotypes. Thirty-eight amplification products of different sizes were recorded across the evaluated landraces and cultivars. Specific PCR profiles were amplified for each obsolete cultivar and landrace and for all evaluated modern Czech cultivars. Analysis revealed differences between modern and obsolete cultivars and landraces in the representation of LMW-GS alleles. High variability was found within evaluated obsolete cultivars and landraces. Sufficient evidence has been gained to suggest that obsolete cultivars and landraces can represent a source of alternative LMW-GS alleles for the wheat breeding programs.
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