Abstract
BackgroundPseudomonas aeruginosa (P. aeruginosa) is prevalent in hospital-acquired surgical wound infections. It exhibits both innate and acquired resistance to a broad range of antimicrobials and remains a principal problem in clinical practice.MethodsIn total, 284 sterile surgical wound swabs (142 each) were collected from two government hospitals: Central Hospital Benin (CHB) and University of Benin Teaching Hospital (UBTH) in Benin City, Nigeria. Pseudomonas spp. isolated from both hospitals were screened with eight different antibiotics by way of disk diffusion method. Polymerase chain reaction (PCR) amplification of 34 multiple drug-resistant isolates was carried out using genus-specific primer set on extracted genomic DNA for the identification of Pseudomonas spp. and substituent 16S rRNA sequencing to determine the prevailing strains in the two locations.ResultsSixty-two Pseudomonas spp. were isolated from the two locations (27 isolates from CHB and 35 isolates from the UBTH). Surgical wound infections screened with regularly used antibiotics revealed that 17 (62.9%) isolates from CHB and 20 (57.1%) isolates from UBTH were multiple drug resistant Pseudomonas spp. PCR identification using Pseudomonas spp. specific primer showed that 16 (94.1%) isolates from CHB and 18 (90%) isolates from UBTH were confirmed. The 16S DNA sequencing revealed that P. aeruginosa strain H25883 was dominant in both locations.ConclusionHigh antibiotic resistance among P. aeruginosa isolates was established in our study. PCR technique revealed a more reliable method of bacterial identification. H25883 strain of P. aeruginosa is the prevalent strain in both locations and it should be given attention in nosocomial surgical wound infections.
Highlights
Post-surgical wound infection is the major source of nosocomial infection in surgical patients, accounting for 39.9% of all infections
Despite the existing and extensive reports on the prevalence of P. aeruginosa in hospital environments, there is still a paucity of research finding on molecular identification of multidrug P. aeruginosa strains from surgical wounds in Benin City, Nigeria
Two hundred and eighty-four postoperative wound swabs specimens were collected from patients in Central Hospital Benin (CHB) and University of Benin Teaching Hospital (UBTH) both in Benin City and analysed
Summary
Post-surgical wound infection is the major source of nosocomial infection in surgical patients, accounting for 39.9% of all infections. It mainly causes post-operative morbidity, resulting in longer hospital stay, increased hospital bill and incidences of postoperative death. A wide range of diagnostic methods have been established for P. aeruginosa identification They include phenotypic methods [10], electrochemical techniques [11] such as enzyme-linked immunosorbent assay [12], and molecular methods such as polimerase chain reaction (PCR) [13], real-time PCR [14, 15], and 16S DNA sequencing [16]. Pseudomonas aeruginosa (P. aeruginosa) is prevalent in hospital-acquired surgical wound infections It exhibits both innate and acquired resistance to a broad range of antimicrobials and remains a principal problem in clinical practice
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