Abstract
Phospholipid (PL) profile was determined on lipids extracted from the whole grains of 10 genotypes of tetraploid wheats ( Triticum dicoccon Schrank and Triticum durum Desf.) and 10 genotypes of hexaploid wheats ( Triticum spelta L. and Triticum aestivum L.) by a liquid chromatography system coupled on-line with an evaporative light scattering detector (HPLC-ELSD). Phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine were the major PL determined, accounting for more than 70% of total PL. PL represented about 10% of total lipids and were detected in naked wheats at levels of 10.1 and 10.4 g/100 g of lipids ( T. durum and T. aestivum, respectively) whereas in the corresponding ancestors T. dicoccon and T. spelta PL amounts were 8.4 and 9.1 g/100 g of lipids, respectively. PL amount in naked wheats was about 17% higher than that determined on average in hulled wheats: 10.2 ± 2.8 vs. 8.7 ± 2.2 g/100 g of lipids, respectively. A hyphenated system HPLC-ELSC was revealed as a useful and more informative tool to identify different PL classes with a minimal experimental workout in comparison to the organic phosphorus assay. The identification of PL molecular species was performed by HPLC, employing as detector a mass spectrometer working in negative ASI-ES mode.
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