Abstract
Eight polymorphic microsatellite loci were isolated from massively parallel next-generation sequencing data and tested in three populations (74 individuals) of the colonial freshwater bryozoan Fredericella sultana. Up to 13 alleles per locus were found and all loci were polymorphic in all populations. Minimum of three loci were sufficient to distinguish all unique multilocus genotypes. These highly variable markers are suitable for clonal identity assignment based on unique multilocus genotypes and provide tools for resolving fine scale population structure in a species characterised by clonal, vegetative growth and asexual reproduction.
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