Abstract
The use of endophytic bacteria in agriculture provides an effective way of improving crop yield and significantly reducing chemical usage, such as fungicides. This research was conducted to explore endophytic bacteria with plant growth promotion (PGP) and antifungal activities against Fusarium moniliforme AIT01. In this study, we obtained 52 isolates of endophytic bacteria associated with the roots and stems of sugarcane from Nakhon Ratchasima province, Thailand. In vitro antagonistic activity test showed that 14 out of 52 isolates had antagonistic activity against the fungal pathogen F. moniliforme AIT01. These antagonistic endophytic bacteria were identified as belonging to six different species as follows: Nguyenibacter vanlangensis, Acidomonas methanolica, Asaia bogorensis, Tanticharoenia aidae, Burkholderia gladioli and Bacillus altitudinis based on phenotypic characteristics, along with phylogenetic analysis of their 16S rRNA gene sequences. Seven isolates effectively inhibited F. moniliforme AIT01 mycelial growth by up to 40%. The volatile compounds of six isolates reduced the growth of F. moniliforme AIT01 by over 23%. Moreover, riceberry rice seedlings previously treated with B. gladioli CP28 were found to strongly reduce infection with phytopathogen by 80% in comparison to the non-treated control. Furthermore, the isolates also showed relevant PGP features, including ammonia production, zinc and phosphate solubilisation, auxin and siderophore biosynthesis. These results demonstrated that the tested endophytic bacteria could be successfully utilised as a source of PGP and biocontrol agent to manage diseases caused by F. moniliforme.
Highlights
Endophytic bacteria spend at least one period of their life cycles inside the tissues of plants without inducing any disease symptoms (Hardoim et al 2015)
In vitro antagonistic activity test showed that 14 out of 52 isolates had antagonistic activity against the fungal pathogen F. moniliforme AIT01. These antagonistic endophytic bacteria were identified as belonging to six different species as follows: Nguyenibacter vanlangensis, Acidomonas methanolica, Asaia bogorensis, Tanticharoenia aidae, Burkholderia gladioli and Bacillus altitudinis based on phenotypic characteristics, along with phylogenetic analysis of their 16S rRNA gene sequences
The isolates showed relevant plant growth promotion (PGP) features, including ammonia production, zinc and phosphate solubilisation, auxin and siderophore biosynthesis. These results demonstrated that the tested endophytic bacteria could be successfully utilised as a source of PGP and biocontrol agent to manage diseases caused by F. moniliforme
Summary
Endophytic bacteria spend at least one period of their life cycles inside the tissues of plants without inducing any disease symptoms (Hardoim et al 2015). Some of them are known as plant growth-promoting bacteria (PGPB) that play roles in agriculture through direct and indirect mechanisms. The biosynthesis of phytohormones, nitrogen fixation, ACC (1-aminocyclopropane-1-carboxylate) deaminase activity, phosphate or zinc solubilisation, and uptake of iron by siderophore production are the direct mechanisms that lead to promoting plant growth. ACC deaminase, cell wall degrading enzymes, hydrogen cyanide, siderophores, induced systemic resistance, and quorum quenching (Olanrewaju et al 2017). Tanticharoenia aidae and Acetobacter sacchari, were the second and third species, respectively, of plant growth promoting bacteria associated with sugarcane (Vu et al 2016; 2019b). Various kinds of bacterial endophytes such as Burkholderia, Raoultella, Ralstonia, Mesorhizobium, Ochrobactrum, Sphingomonas, Novosphingobium, Pantoea and Bacillus were found in sugarcane (Tam & Diep 2014; Muangthong et al 2015; Luo et al 2016; Rezamahalleh et al 2019; Zeng et al 2020; Rocha et al 2021)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
