Abstract
Tuberculosis (TB) is an infectocontagious respiratory disease caused by members of the Mycobacterium tuberculosis complex. A 7 base pair (bp) deletion in the locus polyketide synthase (pks)15/1 is described as polymorphic among members of the M. tuberculosis complex, enabling the identification of Euro-American, Indo-Oceanic and Asian lineages. The aim of this study was to characterise this locus in TB isolates from Mexico. One hundred twenty clinical isolates were recovered from the states of Veracruz and Estado de Mexico. We determined the nucleotide sequence of a ± 400 bp fragment of the locus pks15/1, while genotypic characterisation was performed by spoligotyping. One hundred and fifty isolates contained the 7 bp deletion, while five had the wild type locus. Lineages X (22%), LAM (18%) and T (17%) were the most frequent; only three (2%) of the isolates were identified as Beijing and two (1%) EAI-Manila. The wild type pks15/1 locus was observed in all Asian lineage isolates tested. Our results confirm the utility of locus pks15/1 as a molecular marker for identifying Asian lineages of the M. tuberculosis complex. This marker could be of great value in the epidemiological surveillance of TB, especially in countries like Mexico, where the prevalence of such lineages is unknown.
Highlights
Tuberculosis (TB) is a respiratory infectocontagious disease caused by members of the Mycobacterium tuberculosis complex
There was a significant presence of TB drug-resistance (42%) and multidrug resistance (MDR) (22%) in the recovered isolates, supporting previous observations indicating MDR as a growing problem in Mexico (Quitugua et al 2002, Molina-Torres et al 2010, Nava-Aguilera et al 2011, Zenteno-Cuevas et al 2012)
19% (23) of the population had a medical history of DM-2, coinciding with reports illustrating a high association of this comorbidity within Mexico (Ponce-de-Leon et al 2004, Nava-Aguilera et al 2011, Pérez-Navarro et al 2011, Zenteno-Cuevas et al 2012, Cuevas-Córdoba et al 2013, Jiménez-Corona et al 2013)
Summary
Tuberculosis (TB) is a respiratory infectocontagious disease caused by members of the Mycobacterium tuberculosis complex. The pks15/1 locus has been considered a potential marker for identifying W-Beijing, Asian (non-Beijing) and Indo-Oceanic lineages in countries with a low prevalence of these strains and a significant immigrant online | memorias.ioc.fiocruz.br pks15/1 characterisation TB Roberto Zenteno-Cuevas et al 719 population presence (Alonso et al 2008). Characterisation of this marker is practically unknown in isolates from Latin America. The aim of our study was to characterise the pks15/1 locus in clinical isolates of M. tuberculosis from different locations in Mexico
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