Abstract
BackgroundInterleukin-10 (IL-10) is a negative regulator of immune responses and was previously shown to be expressed by human nasal endothelial cells, while the adhesion molecule MECA-79 plays a role in trans-endothelial migration of immune competent cells. In this study we investigate the relationship between endothelial IL-10 and MECA-79 expression to address the question whether immune competent cells could be affected at the mucosal entry site.MethodsNasal turbinate biopsies were taken from house dust mite allergic patients, before and after nasal allergen provocation. Subsequent slides of biopsies were stained for IL10, MECA-79, CD34, and IL10-Receptor. Capillaries, arteries/veins, and sinusoids were evaluated separately.Results90% of sinusoids are IL-10 positive and all sinusoids are negative for MECA-79, while 4.8% of capillaries are positive for IL-10, and 2.2% are positive for MECA-79. Although about 47% of arteries/veins are positive for IL-10 and 57.1% are positive for MECA-79, only about 20% are positive for both markers. Furthermore, we showed that the myo-fibroblasts surrounding all sinusoids stain positive for IL10R.ConclusionsIL10 expression on vascular structures is not related to MECA expression for sinusoids and capillaries and only partly related on arteries/veins, however sinusoidal endothelial IL10 expression is always seen in combination with IL-10R expression of sinusoidal myo-fibroblasts.
Highlights
Interleukin-10 (IL-10) is a negative regulator of immune responses and was previously shown to be expressed by human nasal endothelial cells, while the adhesion molecule MECA-79 plays a role in trans-endothelial migration of immune competent cells
In order to look into the effect on cell-trafficking, we identified the main cellular entry sites for inflammatory cells in the nasal mucosa by using the MECA-79 antibody which is directed against ligands of L-selectin, We aimed to investigate how these entry sites relate to endothelial IL-10 expression, at baseline as well as after nasal provocation
Most sinusoids are positive for IL-10. These numbers are in contrast with the low level of IL10 expression on capillaries
Summary
Interleukin-10 (IL-10) is a negative regulator of immune responses and was previously shown to be expressed by human nasal endothelial cells, while the adhesion molecule MECA-79 plays a role in trans-endothelial migration of immune competent cells. In this study we investigate the relationship between endothelial IL-10 and MECA-79 expression to address the question whether immune competent cells could be affected at the mucosal entry site. We previously found expression of Interleukin-10 (IL-10) on the endothelial cells in the nasal mucosa of allergic subjects. This endothelial IL-10 expression appeared to have a strong inverse correlation with the induced symptoms of allergic rhinitis [8]. In order to explore this option we investigated what vascular structures could play a role in trans-migration by identifying the distribution of MECA-
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