Abstract
In the present study, we followed qualitatively the release of fibronectin/s (FNs) and of plasminogen activators (PAs) into the serum-free medium of normal and Rous sarcoma virus (RSV) transformed chicken embryo fibroblasts (CEF). It has been found by Western blotting analysis that RSV transformed cells release into the medium intact FN and FN peptides with a Mr ranging between 230 and I10 kDa while uninfected cells and cells infected by NY68 and PAI (temperature sensitive, ts, mutants for transformation), grown at the restrictive temperature for transformation (41°C), release mainly intact FN. The zymographic analysis of the PAs released by normal and transformed cells showed that transformed cells release into the medium a set of PA forms with Mr ranging between 180 and 43kDa while uninfected cells or cells infected with NY68 or PA1 grown at 41°C do not release or release only the 43 kDa form respectively. The analysis of the PAs present in the extracts of uninfected or PAI and NY68 infected cells, grown at 35° or 41°C, revealed mainly the presence of the 43 kDa form.
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