Abstract
Hand, Foot and Mouth Disease (HFMD), a contagious viral disease that commonly affects infants and children with blisters and flu like symptoms, is caused by a group of enteroviruses such as Enterovirus 71 (EV71) and coxsackievirus A16 (CA16). However some HFMD caused by EV71 may further develop into severe neurological complications such as encephalitis and meningitis. The route of transmission was postulated that the virus transmit from one person to another through direct contact of vesicular fluid or droplet from the infected or via faecal-oral route. To this end, this study utilised a human colorectal adenocarcinoma cell line (HT29) with epithelioid morphology as an in vitro model for the investigation of EV71 replication kinetics. Using qPCR, viral RNA was first detected in HT29 cells as early as 12 h post infection (hpi) while viral protein was first detected at 48 hpi. A significant change in HT29 cells’ morphology was also observed after 48 hpi. Furthermore HT29 cell viability also significantly decreased at 72 hpi. Together, data from this study demonstrated that co-culture of HT29 with EV71 is a useful in vitro model to study the pathogenesis of EV71.Electronic supplementary materialThe online version of this article (doi:10.1186/2193-1801-2-267) contains supplementary material, which is available to authorized users.
Highlights
Hand, Foot and Mouth Disease (HFMD), a contagious viral disease that commonly affects infants and children, are caused by a group of enteroviruses such as Enterovirus 71 (EV71) and coxsackievirus A16 (CA16) (Brown et al 1999; Cardosa et al 2003; Lee et al 2009; Prager et al 2003)
To this end a human colorectal adenocarcinoma cell line (HT29) with epithelioid morphology was infected with EV71 over the time course of 72 h
Cytopathic effects and cell viability of HT29 cells during EV71 infection There are no changes between control and infected cells at 12 hpi, 24 hpi and 48 hpi (Figure 1)
Summary
Foot and Mouth Disease (HFMD), a contagious viral disease that commonly affects infants and children, are caused by a group of enteroviruses such as Enterovirus 71 (EV71) and coxsackievirus A16 (CA16) (Brown et al 1999; Cardosa et al 2003; Lee et al 2009; Prager et al 2003). This self-limiting disease is characterised by fever, rashes, poor appetite and multiple ulcers in mouth (Brown et al 1999; Cardosa et al 2003; Lee et al 2009; Prager et al 2003). There are no available antiviral therapies or vaccines approved by the United States Food and Drug Administration (FDA) to prevent HFMD infections (Li et al 2007)
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