Abstract
ABSTRACTThe aim of the present investigation was to characterize enzymes, produced by extremophilic microorganisms in soil samples, collected from different regions in Bulgaria. The cultivation conditions for obtaining high yield of collagenolytic activity were optimized for two active strains—A 35 and E 15. The molecular mass of the active fractions in the cultural liquid of both strains was determined by means of gel chromatography on Sephadex G-100 and were in the range from 40 to 140 kDa. The proteolytic enzymes were stable against high ethanol concentrations, which allows an effective elimination of different byproducts (pigments, nutrient medium components and metabolites). The electrophoretic patterns show a certain isomorphism of the fractions. The comparison with clostridiopeptidase A, as well as the inhibitory analysis show that these enzymes might be considered as “collagenase-like” enzymes. Preliminary experiments show that they could be applied for pre-treatment of collagen-rich raw materials, e.g. in the pelt industry.
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