Abstract
Galactosyl ceramide 3-sulphate (cerebroside 3-sulphate) was tritiated using [3H]NaBH4 with PdCl2 as catalyst. Quantitative purification of the three components of the product was achieved by chromatography on Florisil. Dispersions, prepared by either low energy sonication or by dilution from organic solvent, were compared by controlled pore glass chromatography, ultra-centrifugation and electron microscopy. Both dispersion techniques were shown to form unilamellar vesicles, the average size of vesicles produced by sonication being far larger than those produced by solvent dilution. The diameter of isolated vesicles produced by solvent dilution was in the range 10–80 nm.
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