Abstract
Ca 2+ transport in mitochondria isolated from rat white adipocytes has been examined and many of the properties found to be similar to those reported for mitochondria isolated from rat liver. Ca 2+ transport is ruthenium red-sensitive ( K i ∼5 pmol.mg protein −1), the affinity for free Ca 2+ is high ( K m ∼3.3 μM) and the V max is 135 nmol Ca 2+.min −1.mg protein −1 at 4°C with 0.2 mM P i present. Ca 2+ transport is stimulated by increasing the medium [P i], and is inhibited when ATP or Mg 2+ is added to the incubation system and in contrast to brown adipocyte mitochondria, Ca 2+ efflux is not promoted by Na +. White adipocyte mitochondria may play a rôle in the regulation of total cell calcium in this tissue.
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