Abstract
N-acylhomoserine lactones (AHL) plays roles as signal molecules in quorum sensing (QS) in most Gram-negative bacteria. QS regulates various physiological activities in relation with population density and concentration of signal molecules. With the aim of isolating marine water-borne bacteria that possess QS properties, we report here the preliminary screening of marine bacteria for AHL production using Chromobacterium violaceum CV026 as the AHL biosensor. Strain T33 was isolated based on preliminary AHL screening and further identified by using 16S rDNA sequence analysis as a member of the genus Vibrio closely related to Vibrio brasiliensis. The isolated Vibrio sp. strain T33 was confirmed to produce N-hexanoyl-l-homoserine lactone (C6-HSL) and N-(3-oxodecanoyl)-l-homoserine lactone (3-oxo-C10 HSL) through high resolution tandem mass spectrometry analysis. We demonstrated that this isolate formed biofilms which could be inhibited by catechin. To the best of our knowledge, this is the first report that documents the production of these AHLs by Vibrio brasiliensis strain T33.
Highlights
The discovery of luminescence in the marine bacterium Vibrio fischeri in the late 1970s has paved the way for research into the mechanisms of regulation of bacterial physiological activities by cell-cell communication [1]
We investigated the presence of quorum sensing (QS) bacteria in a Malaysian tropical marine water sample
The bacterial biosensor chosen for the preliminary screening of acyl homoserine lactone (AHL) in this study was Chromobacterium violaceum CV026, which responds by induction of purple violacein pigmentation [19]
Summary
The discovery of luminescence in the marine bacterium Vibrio fischeri in the late 1970s has paved the way for research into the mechanisms of regulation of bacterial physiological activities by cell-cell communication [1]. The bacteria are able to sense the cell density population by tracking the concentration of signal molecules. This phenomenon is named “quorum sensing (QS)” by Fuqua and co-workers [2,3]. In QS, the concentration of signal molecules plays a vital role that reflects the bacterial population density, when at quorate, specific target genes are activated [4] and a collective behavioral adaptation will occur [5]. Research on QS pathogens could be significant in controlling disease besides helping understand their mechanism of pathogenicity This raised significant interest in our group to extend our research on isolating marine bacteria that possess QS properties. QS bacterium, and its unique AHL production which has not been reported before
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