Abstract
Polysaccharides from Viscum album L., which are characterised as arabino-galactans, are contained in aqueous mistletoe preparations. It is known that they have immune modulating properties. In order to improve the pharmaceutical quality it's desirable to characterize and quantify the arabino-galactans in mistletoe preparations. Here we present a simple method to detect hydrolysable saccharides within mistletoe extracts by simultaneous identification of arabinose, fructose, galactose, glucose, rhamnose and xylose. Because arabinose and galactose are typical components of polysaccharides, it is a profound indication for the composition and the amount of arabino-galactan-polysaccharides comparing the quantification after with the amount before hydrolysis. The monosaccharides are precolumn derivatized with benzocaine by reductive amination to a secondary amine which absorbs at 306nm. Baseline separation is obtained within 90min by HPLC on a Phenomenex aqua column. The quantification of monosaccharides before and after hydrolysis displays the composition and the concentration of hydrolysable saccharides, whereas no information on the total size is gained.
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