Characterisation and Analysis of Chitosan Extract from Artemia franciscana Using SEM, FT-IR and XRD Studies

Abstract

Introduction: Artemia franciscana is one of the best animal models which is used in various fields. After the hatching of Artemia the shell wastes are used for Chitosan extraction. The extraction process consumes less time when compare with other shells because of its shell size.
 Materials and Methods: The Chitosan extract was prepared using Artemia shells. The Physico-chemical properties were analysed and characterization of Chitosan extract was done using SEM, XRD and FT-IR studies.The physicochemical properties of the extracted chitosan indicate that it has optimum values desirable for wound healing applications. Chitosan has high water and fat binding capacity that can help to maintain a moist environment on the wound surface and control inflammation, respectively. The degree of deacetylation (DD) of chitosan is critical in determining its biological activities. The DD value obtained in this study was 84.77%, indicating that the chitosan sample had a high degree of deacetylation. Outcomes: The morphology of the chitosan membrane is an important factor that influences its properties and potential applications, including wound healing.
 Results: The SEM images obtained in this study revealed that pure chitosan exhibited a nonporous, smooth membranous phase with dome-shaped orifices, microfibrils, and crystallites. The XRD analysis results suggest that the chitosan used in this study is highly crystalline, with both crystalline and amorphous regions enhancing its bioactivity and biocompatibility, making it a promising material for wide range of application in medicine. The crystalline and amorphous structure of Chitosan is analysed by the XRD, SEM and FT-IR reveals its wide range of applications in medicine.