Abstract
The advantages and disadvantages of the most common methods for long-term preservation of cultures of fungal entomopathogens—serial transfer, mineral oil, distilled water stasis, silica gel, freeze-drying (lyophilization) and cryopreservative techniques—are discussed, and protocols for these techniques are provided. The choice among so many differing preservation techniques is often difficult but should be based on a given method’s suitability to a laboratory’s realistic needs to preserve fungi and on available technological and financial capacities. Several other less commonly used, inexpensive, and simply applied preservation techniques (whose utility may or may not be confirmed with fungal entomopathogens) are also noted.
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