Abstract
Reversed phase high-performance liquid chromatography (HPLC) is utilized for the separation of molecules due to their polarity in order to quantify, identify, and/or purify various samples such as those from serum, human and animal tissues, drugs, and foods. The following protocols are for extracting carotenoids from samples and subsequent HPLC analysis. If you are interested in another compound for HPLC analysis, Sigma-Aldrich® has a wonderful online resource for multiple adaptations to the HPLC protocol for the analysis of hundreds of compounds (see References).
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