Abstract
The level of virus-neutralizing antibodies (VNA) against rabies virus (RABV) glycoprotein (G) plays a crucial role in protection against rabies. Functional assays, such as the rapid fluorescent focus inhibition test (RFFIT) and the fluorescent antibody virus neutralization test (FAVN), are suitable for measuring the VNA levels in human and animal sera in the laboratory. However, these assays are time-consuming, expensive, and require live RABV, which means that they can only be performed in restricted reference laboratories. Based on the principle of immunochromatography and the VNA-binding activity of inactivated virus, we developed an immunochromatographic strip test called the “Rapid Neutralizing Antibody” (RAPINA) test for the qualitative or semi-quantitative determination of antibody levels. The theoretical basis of the RAPINA test is that the inactivated CVS-11 RABV binds with antibodies in the serum and is no longer detected by the immunochromatography strip, which detects G, so a band is not produced on the test strip.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Current Laboratory Techniques in Rabies Diagnosis, Research and Prevention
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.