Abstract
Since the 1990s, we have witnessed a steady progress in solid-state 17 O NMR spectroscopy for biomolecules. Up until today, majority of the reported studies are considered as preliminary works, but significant, for laying the foundations of 17 O NMR applications to biological solids. This has taken over 20 years with nearly 100 studies in methodology developments and NMR assessments. Currently, the field is entering an exciting stage where small biomolecules can routinely be characterized and new area of research can be exploited, such as investigations of macromolecules and multi-dimensional correlation experiments. This review provides a summary of the important contributions and millstones in the field, including the methodologies for probing the dilute 17 O-spin in biomolecules and the investigations of the nuclear properties in relationships to the oxygen local surroundings.
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