Abstract
Publisher Summary This chapter discusses the macroscopic-scale repulsion versus microscopic-scale attraction in water, methodologies used in measuring protein adsorption onto and desorption from metal oxide particles in water, hysteresis of protein adsorption onto metal oxide surfaces in water, and kinetics of protein adsorption onto metal oxide surfaces immersed in water. In the interaction between human serum albumin (HSA) molecules dissolved in water, and silica particles (SiO 2 ) suspended in an HSA solution, it is shown that the overall macroscopic-scale repulsion between HAS molecules and silica particles is ≈5.1 times greater than the microscopic-scale attraction between the HSA molecules and discrete cationic sites on the SiO 2 particles. Given the necessity of maintaining the adsorbing particles in an unhindered suspension, a device is constructed which used a closed-circuit aqueous circulation of the (in this case HSA) solution which passed through a (magnetically stirred) compartment of suspended metal oxide particles, enclosed between two microporous (Nuclepore) membranes with multiple monosized pores which are just small enough to prevent the particles from escaping.
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