Abstract
External murine ears collected postmortem, as well as ear punches obtained during standard marking of live animals, are the source of mesenchymal stem cells, termed ear mesenchymal stem cells (EMSC). These cells provide an easily obtainable, primary culture model system for the study of lineage commitment and differentiation. EMSC are capable of differentiating into adipocytes, osteocytes, chondrocytes, and contractile myocytes. Facile adipogenic differentiation of EMSC provides an excellent model for the study of adipogenesis. In this chapter, methods for isolation, culture, and differentiation of EMSC are described.
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