Abstract
Genome-wide investigation of DNA methylation is of great interest to fields of biological sciences and medicine. Reduced representation bisulfite sequencing (RRBS) is a bisulfite-based method that combines a restriction enzyme (e.g. MspI) cut to enrich for CG-dense regions in the genome followed by next-generation sequencing for profiling global DNA methylation at single-base resolution. Compared to whole genome bisulfite sequencing, RRBS reduces more than 95% of sequencing requirement for capturing CpGs of ~80% CpG islands, more than 50% promoters, as well as relative smaller percentage of other genomic regulatory regions, such as enhancers, in human genome. It has been extensively applied to researches in mammalian development/differentiation, aging, environmental biology, and human disease, such as cancer. RRBS, among a variety of global DNA methylation mapping approaches, represents a sensitive, cost-effective, high throughput method suitable for the assessment of genome-wide methylation profiles of a large sample size, including clinical samples.
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