Abstract
Publisher Summary This chapter illustrates the variety of ways in which monoclonal antibody (MAb) technology can be used for the analysis of cell- and tissue-specific carbohydrate antigens present at the surfaces of plant cells. MAbs to cell-specific cell surface carbohydrate epitopes are versatile molecular probes with multiple applications for plant cell biology and development, for plant molecular biology, and for plant cell and tissue culture. MAbs have been essential probes for identifying and characterizing developmentally regulated cell surface epitopes in plants, and now can be used for the manipulation of the cells in which they are expressed. The chapter focuses on the use of MAbs for the purification and characterization of cell- and tissue-specific carbohydrate antigens and for the manipulation of defined cellular targets. Indirect immunolabeling techniques are used in this chapter. The MAb is first bound to the antigen then, after washing to remove surplus MAb, is tagged with an enzyme, radioisotope, fluorochrome, electron-opaque particle, or paramagnetic bead. The chapter illustrates the variety of ways in which MAb technology can be used for the analysis of cell- and tissue-specific carbohydrate antigens present at the surfaces of plant cells.
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