Abstract
This chapter describes the methods for isolation of nuclei and chromatin from Phycomyces blakesleeanus. Phycomyces blakesleeanus is studied as a model sensory system. The giant unicellular sporangiophore is the most remarkable organelle in the Phycomyces . It exhibits considerable regenerative powers. The methodology used in laboratory to grow mycelia and sporangiophores and to isolate intact nuclei from the sporangiophores includes (1) growth of Phycomyces blakesleeanus , (2) isolation of nuclei from sporangiophores, and (3) isolation of chromatin. Control of the development of the sporangiophore at the level of gene expression may be relatively simple. Chromatin isolated from various stages of synchronized sporangiophores contains nonhistone chromosomal proteins that exhibit considerable differences, both quantitative and qualitative, during sporangiophore development. Extracted chromatin contains large quantity of histones including H1, H2B, H2A, and H4.
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