Abstract
Publisher Summary This chapter describes the immunochemical analysis of nonhistone proteins. In contrast to histones, the heterogeneous mixtures of nonhistone proteins are very good immunogens. To produce antibody, nonhistone proteins can be injected into the experimental animal complexed with DNA. Administration of whole chromatin also has been shown to give antibody against nonhistone proteins as well as against histones. Free as well as sodium dodecyl sulfate (SDS)-treated nonhistone protein preparations are good immunogens. No difficulties have been reported in producing antibody against nonhistone proteins from various eukaryotes. The chapter introduces the methodology of immunochemistry of chromosomal nonhistone proteins. It also reviews some applications of the immunochemical analysis in characterization of the nonhistone proteins as well as in characterization of chromatin. The material discussed in the chapter focuses on antibody raised against heterogeneous mixtures of these proteins. Immunogenicity of nonhistone proteins is described. The chapter discusses the choice of animal for production of antihistone antibody and presents an overview of immunization procedure.
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