Abstract
Publisher Summary Flow cytometry is a sophisticated, quantitative technique with many applications in biomedical research and clinical medicine, and can be applied to examine large numbers of individual cells, allowing rapid measurement of as many as eight different cellular parameters per cell, such as cell size, or light scatter. This chapter explores the data from flow cytometric analyses of several neural cell lines, which are used as alternate sources of donor tissue for transplantation and for the analyses of the developing rat hypothalamus. The chapter also discusses the preliminary results of electronically sorted neurons for transplantation and the requirements for obtaining these cells in an optimal condition. Flow cytometry has the potential to provide important analytical data on surface properties of transplantable cells, and surface glycoconjugates, such as specific glycoproteins and membrane fluidity measurements of differentiated cells have been quantified for several clonal neural cell lines. However, to obtain relatively rare populations of viable neurons for transplantation, retrograde transport of fluorescent markers to discrete CNS neurons followed by isolation by cell sorting is an experimental method, which has promise, and can be employed in transplantation studies.
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