Abstract
Persistent reliance on the cost-intensive second-generation biofuel production mainly depends on the enzymatic saccharification efficiencies of lignocellulosic waste biomasses. Lignocellulosic wastes are the most abundantly available and renewable carbon sources on the earth, having immense capacity to substitute fossil fuels on the basis of economic and eco-friendly biofuel processing. However, enzyme hydrolysis of lignocellulosic biomass is still not considered cost-effective due to expensive nature of cellulase enzyme and inappropriate utilization strategy of the enzyme. Strategies like production of potent enzymes from waste biomasses while implementing high-yield microbes, developing the ideal environmental condition to improve catalytic activity of the enzyme, implementing ideal process conditions like fed-batch saccharification for improved production of reducing sugar, and reutilization strategies of enzymes play the most important roles in making the overall enzymatic hydrolysis process sustainable, environment friendly, and finally, it can effectively contribute in framing economic lignocellulosic biofuel production process. This chapter clearly elaborates on such important aspects associated with enzymatic hydrolysis process of lignocellulosic biomass.
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