Abstract
Publisher Summary This chapter describes the molecular and genetic methods for studying mitosis and spindle proteins in Aspergillus nidulans . The fungus A. nidulans is one of the most promising organisms being used to study the molecular biology of mitosis. The special utility of Aspergillus is that it has a sophisticated and powerful genetic system. A. nidulans is easy to handle using conventional microbiological techniques, and it grows on simple, inexpensive media to high densities, to provide ample material for biochemical analysis. Mitotic index is usually defined as the percentage of nuclei in mitosis, but A. nidulans is coenocytic and nearly all nuclei within a hyphal segment enter mitosis at the same time. Genetic mapping in A. nidulans is usually done in two stages. Mutations are first mapped to linkage group by the parasexual method. Proteins and nucleic acids can be readily labeled in vivo by germinating conidiospores in a liquid minimal medium supplemented for nutritional requirements and containing a radioactively-labeled precursor.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
