Abstract
This chapter focuses on isotope ratio mass spectrometry (IRMS). IRMS determines the ratio of isotopes of a given element, such as H: 2 H and 12 C: 13 C present in a specimen. The total amount of each isotope also is determined. The amount of element derived from labeled drug in a specimen can be computed from the excess in labeled element isotope ratio and the total amount of element. IRMS offers the potential of detecting and quantitating added label in any whole biological matrix. It also offers simplicity and flexibility similar to radioactive tracer procedures without the administrative and safely concerns of radioactive studies. In continuous flow-isotope ratio mass spectrometry (CF-IRMS), organic sample is introduced into the high temperature combustion chamber of an elemental analyzer and flash combusted with a pulse of oxygen. The oxidized combustion gases (CO 2 , N 2 , and H 2 O) are carried by a continuous stream of helium through clean-up phases to a gas chromatography-isotope ratio mass spectrometry (GC-IRMS) device.
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