Abstract
It does not happen very often in a science history when a single discovery becomes a technological key stone, especially in Biology. CRISPRs discovery and a path to its application in Genetic Engineering is a perfect example of such breakthrough, which is comparable to the discovery of the polymerase chain reaction or development of the next generation sequencing technology. To date, there are a few known defensive strategies in Archaea and Bacteria that can be referred to as the multilayer prokaryotic immune system: the restriction-modification system (RMS), the adsorption inhibition, abortive infection, blocking DNA injection, and the recently discovered Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs). In this book chapter, I discuss the history of the CRISPR/Cas research, genetic structure of the CRISPR/Cas systems, their molecular mechanisms of action and regulation, as well as unsolved CRISPR/Cas puzzles that will bother minds of scientists.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
