Abstract
This chapter presents an examination of direct smears. Direct microscopical examination of pathological material for acid-fast bacilli may be a valuable or quite useless procedure depending on the nature and quality of the specimen. The microscopical examination of direct smears of sputum is probably the most useful test in the diagnosis and control of pulmonary tuberculosis as it permits the rapid detection of infectious, and the monitoring of the progress of the disease and its treatment. The smears may be made of sputum in its natural state or of the centrifuged deposit after it has been digested with various reagents. To make the smears, the slides should be placed on glass or ceramic tiles, preferably black, or on pieces of plain paper. The tiles are disinfected or the paper incinerated after each batch of slides has been made. In some laboratories, the slides are placed on special racks or slide holders while the smears are made.
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