Abstract
Publisher Summary This chapter describes the biosynthesis and regulation of fatty acid in eucaryotes and focuses on the regulation of fatty acid synthesis in liver or adipose tissue. The chapter also discusses four stages in the analysis of the regulation of a metabolic pathway, including an identification of regulatory enzymes or an analysis of the physical, kinetic, and regulatory properties of those enzymes. Regulation of the genes for the lipogenic enzymes by triiodothyronine, insulin, and cAMP make them part of a small set of identified proteins regulated by these agents. Site-directed mutagenesis coupled with over-expression in bacterial or animal cells will permit direct analysis of the importance of various residues to the regulation of enzyme activity by allosteric and covalent modification mechanisms. Re-expression of mutant acetyl- coenzyme A (CoA) carboxylases altered by site-directed mutagenesis should permit analysis of both allosteric and covalent modification mechanisms under physiological conditions in intact cells and animals.
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