Abstract
Publisher Summary Flow cytometry (FCM) has provided an opportunity to assay single cells and the ability to correlate multiple parameters in a cell population. It enables an analysis of the immunological system in response to the viral infection. All of the analyses have been performed with the flow cytometer utilizing a number of staining reactions. This chapter reviews the protocol that laboratory has utilized to study the permissive and nonpermissive infection of cells with Simian virus 40 (SV40). The chapter outlines the staining protocol for SV40 T antigen in cells cultured in monolayer and stained with propidium iodide for DNA content. This technology can be applied to other viral model systems to obtain information on various parameters of pathogenesis at the cellular level that might apply to the clinical disease. Results have indicated that the technique could be used as both a rapid diagnostic test and to study the pathogenesis of human infections such as herpes simplex virus (HSV-I), cytomegalovirus (CMV), and human immunodeficiency virus (HIV).
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