Abstract
Cilia are thin cellular extensions specialized for motility and/or sensing. Their microtubule-based framework is universally nucleated from a basal body. Between the full-fledged cilium and the basal body lies a specialized region called the transition zone (TZ) made up of unique proteins and distinct structures. Defects in TZ proteins can result in aberrant signaling because of improper trafficking between the cilium and cell body, which in turn can cause a variety of cilia-related diseases (ciliopathies) in humans. The TZ of Chlamydomonas displays an intricate geometry and more details of its structure are emerging with the application of cryo-EM and ultrastructure expansion microscopy. Analysis of cilia isolated from Chlamydomonas mutants in genes for ciliopathy-related TZ proteins show that these proteins are important for regulating the protein composition of the ciliary membrane and matrix. Applying these techniques to additional mutants should lead to a better understanding of the role of the TZ in forming the gateway to the cilium.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
