Abstract
Size-exclusion chromatography is also called gel-filtration or gel-permeation chromatography. This method uses porous particles to separate molecules of different sizes. It is generally used to separate biological molecules and to determine molecular weights and molecular weight distributions of polymers, and the separation of molecules is called fractionation. It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers. The size of the pores in the beads determines the exclusion limit (what goes through the beads and what goes around the beads). There are different types of methods to separate the molecules based on different conditions, such as temperature variation. The usage of size-exclusion chromatography for different applications is also presented.
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