Abstract
Deoxyribonucleases (DNases) occur in prokaryotes, in the nucleus, and in cytoplasmofeukaryotes. Eukaryotic nuclear DNases have been found in the nucleoplasm, in the nucleolus and are associated with chromatin and—in particular—with the nonhistone chromatin proteins. A number of different methods for assaying DNase activity are described in this chapter. Many of these assays detect specific types of DNases whereas others have more general applications. Both endonuclease and exonuclease activity can be determined using methods that measure liberation of acid-soluble material from the substrate. A sensitive DNase assay uses alkaline sucrose gradient centrifugation of DNA preincubated with the enzyme. Most of the studies cited in this chapter assayed DNases isolated from animal tissues or from rapidly growing microbial cultures. An assay permits detection of DNase activity at levels lower than those previously reported. In this method the priming activity of DNA for DNA polymerase is increased by mild treatment of DNA with pancreatic DNase.
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