Abstract
Publisher Summary This chapter discusses functional measurements using HL-60 cells. Exposure of HL-60 cells to a variety of agents induces terminal differentiation along either of two distinct pathways—one that bears many of the surface properties and functional characteristics of mature neutrophils and the other that resembles characteristics of monocytes/macrophages. HL-60 cells show some unique light scatter changes with differentiation. The chapter shows this change as there is a dramatic reduction in cell size and increase in 90° light scatter. As spontaneous differentiation is continually occurring, some differentiated cells are always present in so-called “undifferentiated” populations. A rapid method for checking the differentiation status of HL-60 cells is to evaluate the esterase content. Undifferentiated cells have reasonably high esterase content, which decreases in granulocytic differentiation and increases in monocytic differentiation. Differentiated HL-60 cells also express increased numbers of cell receptors such as CDllb.
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