Abstract
Fluorescence two-dimensional difference gel electrophoresis (2D-DIGE) is a recently developed 2D gel-based proteomics technique that provides a sensitive, rapid, and quantitative analysis of differential protein expression between biological samples. Resolved, labeled proteins are then detected at appropriate excitation and emission wavelengths using a multi-wavelength fluorescence detection device and the signals compared. As well as reducing the number of gels that need to be run, differential labeling and mixing mean that samples are subjected to the same handling procedures and microenvironments during 2D separation, raising the confidence with which protein changes can be detected and quantified. The same gels are then poststained and proteins of interest are picked for MS identification. 2D-DIGE is applicable for the analysis of total cell lysates and complex protein mixtures from cultured cells, whole tissues, sorted or fractionated cells, whole organisms, cellular subfractions, or affinity-purified protein fractions. Thus, the problem of gel-to-gel variation is avoided. This type of single gel experiment is useful where only limited sample quantities are available.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.