Abstract
Publisher Summary There are large collections of human and animal nervous tissue in Neuropathology/Pathology and Neuroanatomy, as well as Neuroscience Departments all over the world. This tissue was originally prepared for microscopic studies and is generally considered of little use for research in molecular genetics because fixation and especially storage in formalin, often followed by paraffin-embedding have damaging effects on DNA. However, using human brain tissue from morphologically proven cases of Parkinson and Alzheimer diseases, this chapter demonstrates that archival tissue specimens, some stored for more than 80 years, may be employed for genotyping as well as genomic sequencing. The conceptual background of this work as well as some of the critical technical differences compared to molecular genetic work involving freshly isolated DNA are discussed here. It is suggested that retrospective genotyping of human and experimental animal tissue will be of increasing relevance for genotype-phenotype analyses in the “postgenomic” era.
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