Abstract
In the present era, with the increase in human population and the demand for food and materials, it is necessary to improve crop production with the implementation of efficient and fast methods. Although the transgenic approach for crop improvement is an efficient method compared to breeding methods, the presence of tissue culture-based generation of transformants is still a hurdle for the successful transformation of many hardy crops. Therefore, the approach of in planta transformation for raising transgenics is a better way for such recalcitrant crops. In planta transformation is fast, more efficient, and a tissue culture-independent-based transformation method for crop improvement. It has more advantage to those crops that lack regeneration and tissue culture systems. This chapter summarizes the major methods of plant transformation, the advantages of in planta transformation over the tissue culture method, and the role of this method in the advancement of crop plants until now. The application of in planta transformation is not bound with crop improvement only. With the development of science and technology, plant transformation has become an important tool in understanding basic plant biology. The application of such a fast and efficient method will increase the pace of understanding the major biological aspects in terms of plants and their improvement.
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